What impact does low viscosity of a solvent have in chromatography?

What impact does low viscosity of a solvent have in chromatography?

• A. Facilitates better migration
• B. Increases retention time
• C. Causes saturation of spots
• D. Decreases boiling point

Answer: (A)

In chromatography, the viscosity of a solvent plays a crucial role in the efficiency and effectiveness of the separation process. Low viscosity solvents allow for faster movement of the mobile phase through the stationary phase. This is because low viscosity substances experience less internal resistance when they flow, which in turn enhances the overall migration of the analytes. With better migration, the separation of components is more pronounced and can yield sharper, more defined spots on the chromatogram. Furthermore, with improved mobility, the distance traveled by the solutes is generally increased, promoting a more effective separation. Faster elution times also contribute to a reduced risk of band broadening, leading to higher resolution between different components. While other choices touch upon important concepts regarding chromatography, they do not directly pertain to the benefits provided by a low viscosity solvent in terms of enhanced migration and separation efficiency.

In chromatography, the viscosity of a solvent plays a crucial role in the efficiency and effectiveness of the separation process. Low viscosity solvents allow for faster movement of the mobile phase through the stationary phase. This is because low viscosity substances experience less internal resistance when they flow, which in turn enhances the overall migration of the analytes. With better migration, the separation of components is more pronounced and can yield sharper, more defined spots on the chromatogram.

Furthermore, with improved mobility, the distance traveled by the solutes is generally increased, promoting a more effective separation. Faster elution times also contribute to a reduced risk of band broadening, leading to higher resolution between different components.

While other choices touch upon important concepts regarding chromatography, they do not directly pertain to the benefits provided by a low viscosity solvent in terms of enhanced migration and separation efficiency.